human il 18 Search Results


elisa kit  (R&D Systems)
94
R&D Systems elisa kit
Figure 3. Silencing p62 reduces IL‑18 secretion and promotes cell survival of macrophages with prolonged oxLDL exposure. (A) THP‑M cells treated with oxLDL (20 µg/ml) for the indicated time following transfection with 50 nM con siRNA or p62 siRNA. Western blotting experiments were performed on total protein extracts using anti‑p62 antibody, and β‑actin expression was used as a loading control. The graph represents the values of p62 band intensity after normalization to β‑actin by densitometry. *P<0.05 vs. 24 h group. (B) The graph represents values of the SQSTM1 mRNA expression following normalization for β‑actin mRNA by reverse transcription‑quantitative polymerase chain reaction. *P<0.05 vs. 24 h group. (C) <t>ELISA</t> of secreted IL‑18 in the media of cells treated with con or p62 siRNA and 20 µg/ml oxLDL for 0, 12, 24, 48 and 72 h. *P<0.05 and **P<0.01 vs. 0 h; ##P<0.01 as indicated. (D) THP‑M cells were pretreated with con or p62 siRNA and then treated with 20 µg/ml oxLDL for the indicated times. Cell viability was analyzed by the MTT assay. Results are expressed as a percentage of con siRNA group at 0 h. *P<0.05 vs. con siRNA group. (E) Determination of cell death by flow cytometry of Annexin V‑fluorescein isothiocyanate/PI staining in THP‑M cells transfected with con or p62 siRNA and then incubated with oxLDL (20 µg/ml) for the indicated times. Data are representatives of three independent experiments. **P<0.01 as indicated. <t>IL,</t> <t>interleukin;</t> oxLDL, oxidized low‑density lipoprotein; THP‑M, THP‑1‑derived macrophages; siRNA, small interfering RNA; con, control; PI, propidium iodide.
Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat anti il 18bp  (R&D Systems)
93
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Figure 3. Silencing p62 reduces IL‑18 secretion and promotes cell survival of macrophages with prolonged oxLDL exposure. (A) THP‑M cells treated with oxLDL (20 µg/ml) for the indicated time following transfection with 50 nM con siRNA or p62 siRNA. Western blotting experiments were performed on total protein extracts using anti‑p62 antibody, and β‑actin expression was used as a loading control. The graph represents the values of p62 band intensity after normalization to β‑actin by densitometry. *P<0.05 vs. 24 h group. (B) The graph represents values of the SQSTM1 mRNA expression following normalization for β‑actin mRNA by reverse transcription‑quantitative polymerase chain reaction. *P<0.05 vs. 24 h group. (C) <t>ELISA</t> of secreted IL‑18 in the media of cells treated with con or p62 siRNA and 20 µg/ml oxLDL for 0, 12, 24, 48 and 72 h. *P<0.05 and **P<0.01 vs. 0 h; ##P<0.01 as indicated. (D) THP‑M cells were pretreated with con or p62 siRNA and then treated with 20 µg/ml oxLDL for the indicated times. Cell viability was analyzed by the MTT assay. Results are expressed as a percentage of con siRNA group at 0 h. *P<0.05 vs. con siRNA group. (E) Determination of cell death by flow cytometry of Annexin V‑fluorescein isothiocyanate/PI staining in THP‑M cells transfected with con or p62 siRNA and then incubated with oxLDL (20 µg/ml) for the indicated times. Data are representatives of three independent experiments. **P<0.01 as indicated. <t>IL,</t> <t>interleukin;</t> oxLDL, oxidized low‑density lipoprotein; THP‑M, THP‑1‑derived macrophages; siRNA, small interfering RNA; con, control; PI, propidium iodide.
Goat Anti Il 18bp, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant human il 18bpa fragment crystallizable region fc chimera  (R&D Systems)
93
R&D Systems recombinant human il 18bpa fragment crystallizable region fc chimera
Figure 3. Silencing p62 reduces IL‑18 secretion and promotes cell survival of macrophages with prolonged oxLDL exposure. (A) THP‑M cells treated with oxLDL (20 µg/ml) for the indicated time following transfection with 50 nM con siRNA or p62 siRNA. Western blotting experiments were performed on total protein extracts using anti‑p62 antibody, and β‑actin expression was used as a loading control. The graph represents the values of p62 band intensity after normalization to β‑actin by densitometry. *P<0.05 vs. 24 h group. (B) The graph represents values of the SQSTM1 mRNA expression following normalization for β‑actin mRNA by reverse transcription‑quantitative polymerase chain reaction. *P<0.05 vs. 24 h group. (C) <t>ELISA</t> of secreted IL‑18 in the media of cells treated with con or p62 siRNA and 20 µg/ml oxLDL for 0, 12, 24, 48 and 72 h. *P<0.05 and **P<0.01 vs. 0 h; ##P<0.01 as indicated. (D) THP‑M cells were pretreated with con or p62 siRNA and then treated with 20 µg/ml oxLDL for the indicated times. Cell viability was analyzed by the MTT assay. Results are expressed as a percentage of con siRNA group at 0 h. *P<0.05 vs. con siRNA group. (E) Determination of cell death by flow cytometry of Annexin V‑fluorescein isothiocyanate/PI staining in THP‑M cells transfected with con or p62 siRNA and then incubated with oxLDL (20 µg/ml) for the indicated times. Data are representatives of three independent experiments. **P<0.01 as indicated. <t>IL,</t> <t>interleukin;</t> oxLDL, oxidized low‑density lipoprotein; THP‑M, THP‑1‑derived macrophages; siRNA, small interfering RNA; con, control; PI, propidium iodide.
Recombinant Human Il 18bpa Fragment Crystallizable Region Fc Chimera, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human il 18  (Elabscience Biotechnology)
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Elabscience Biotechnology human il 18
Figure 3. Silencing p62 reduces IL‑18 secretion and promotes cell survival of macrophages with prolonged oxLDL exposure. (A) THP‑M cells treated with oxLDL (20 µg/ml) for the indicated time following transfection with 50 nM con siRNA or p62 siRNA. Western blotting experiments were performed on total protein extracts using anti‑p62 antibody, and β‑actin expression was used as a loading control. The graph represents the values of p62 band intensity after normalization to β‑actin by densitometry. *P<0.05 vs. 24 h group. (B) The graph represents values of the SQSTM1 mRNA expression following normalization for β‑actin mRNA by reverse transcription‑quantitative polymerase chain reaction. *P<0.05 vs. 24 h group. (C) <t>ELISA</t> of secreted IL‑18 in the media of cells treated with con or p62 siRNA and 20 µg/ml oxLDL for 0, 12, 24, 48 and 72 h. *P<0.05 and **P<0.01 vs. 0 h; ##P<0.01 as indicated. (D) THP‑M cells were pretreated with con or p62 siRNA and then treated with 20 µg/ml oxLDL for the indicated times. Cell viability was analyzed by the MTT assay. Results are expressed as a percentage of con siRNA group at 0 h. *P<0.05 vs. con siRNA group. (E) Determination of cell death by flow cytometry of Annexin V‑fluorescein isothiocyanate/PI staining in THP‑M cells transfected with con or p62 siRNA and then incubated with oxLDL (20 µg/ml) for the indicated times. Data are representatives of three independent experiments. **P<0.01 as indicated. <t>IL,</t> <t>interleukin;</t> oxLDL, oxidized low‑density lipoprotein; THP‑M, THP‑1‑derived macrophages; siRNA, small interfering RNA; con, control; PI, propidium iodide.
Human Il 18, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human il 18 elisa kit  (R&D Systems)
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Figure 3. Silencing p62 reduces IL‑18 secretion and promotes cell survival of macrophages with prolonged oxLDL exposure. (A) THP‑M cells treated with oxLDL (20 µg/ml) for the indicated time following transfection with 50 nM con siRNA or p62 siRNA. Western blotting experiments were performed on total protein extracts using anti‑p62 antibody, and β‑actin expression was used as a loading control. The graph represents the values of p62 band intensity after normalization to β‑actin by densitometry. *P<0.05 vs. 24 h group. (B) The graph represents values of the SQSTM1 mRNA expression following normalization for β‑actin mRNA by reverse transcription‑quantitative polymerase chain reaction. *P<0.05 vs. 24 h group. (C) <t>ELISA</t> of secreted IL‑18 in the media of cells treated with con or p62 siRNA and 20 µg/ml oxLDL for 0, 12, 24, 48 and 72 h. *P<0.05 and **P<0.01 vs. 0 h; ##P<0.01 as indicated. (D) THP‑M cells were pretreated with con or p62 siRNA and then treated with 20 µg/ml oxLDL for the indicated times. Cell viability was analyzed by the MTT assay. Results are expressed as a percentage of con siRNA group at 0 h. *P<0.05 vs. con siRNA group. (E) Determination of cell death by flow cytometry of Annexin V‑fluorescein isothiocyanate/PI staining in THP‑M cells transfected with con or p62 siRNA and then incubated with oxLDL (20 µg/ml) for the indicated times. Data are representatives of three independent experiments. **P<0.01 as indicated. <t>IL,</t> <t>interleukin;</t> oxLDL, oxidized low‑density lipoprotein; THP‑M, THP‑1‑derived macrophages; siRNA, small interfering RNA; con, control; PI, propidium iodide.
Human Il 18 Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti human il18  (R&D Systems)
93
R&D Systems anti human il18
Figure 3. Silencing p62 reduces IL‑18 secretion and promotes cell survival of macrophages with prolonged oxLDL exposure. (A) THP‑M cells treated with oxLDL (20 µg/ml) for the indicated time following transfection with 50 nM con siRNA or p62 siRNA. Western blotting experiments were performed on total protein extracts using anti‑p62 antibody, and β‑actin expression was used as a loading control. The graph represents the values of p62 band intensity after normalization to β‑actin by densitometry. *P<0.05 vs. 24 h group. (B) The graph represents values of the SQSTM1 mRNA expression following normalization for β‑actin mRNA by reverse transcription‑quantitative polymerase chain reaction. *P<0.05 vs. 24 h group. (C) <t>ELISA</t> of secreted IL‑18 in the media of cells treated with con or p62 siRNA and 20 µg/ml oxLDL for 0, 12, 24, 48 and 72 h. *P<0.05 and **P<0.01 vs. 0 h; ##P<0.01 as indicated. (D) THP‑M cells were pretreated with con or p62 siRNA and then treated with 20 µg/ml oxLDL for the indicated times. Cell viability was analyzed by the MTT assay. Results are expressed as a percentage of con siRNA group at 0 h. *P<0.05 vs. con siRNA group. (E) Determination of cell death by flow cytometry of Annexin V‑fluorescein isothiocyanate/PI staining in THP‑M cells transfected with con or p62 siRNA and then incubated with oxLDL (20 µg/ml) for the indicated times. Data are representatives of three independent experiments. **P<0.01 as indicated. <t>IL,</t> <t>interleukin;</t> oxLDL, oxidized low‑density lipoprotein; THP‑M, THP‑1‑derived macrophages; siRNA, small interfering RNA; con, control; PI, propidium iodide.
Anti Human Il18, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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d044 3  (R&D Systems)
92
R&D Systems d044 3
Figure 3. Silencing p62 reduces IL‑18 secretion and promotes cell survival of macrophages with prolonged oxLDL exposure. (A) THP‑M cells treated with oxLDL (20 µg/ml) for the indicated time following transfection with 50 nM con siRNA or p62 siRNA. Western blotting experiments were performed on total protein extracts using anti‑p62 antibody, and β‑actin expression was used as a loading control. The graph represents the values of p62 band intensity after normalization to β‑actin by densitometry. *P<0.05 vs. 24 h group. (B) The graph represents values of the SQSTM1 mRNA expression following normalization for β‑actin mRNA by reverse transcription‑quantitative polymerase chain reaction. *P<0.05 vs. 24 h group. (C) <t>ELISA</t> of secreted IL‑18 in the media of cells treated with con or p62 siRNA and 20 µg/ml oxLDL for 0, 12, 24, 48 and 72 h. *P<0.05 and **P<0.01 vs. 0 h; ##P<0.01 as indicated. (D) THP‑M cells were pretreated with con or p62 siRNA and then treated with 20 µg/ml oxLDL for the indicated times. Cell viability was analyzed by the MTT assay. Results are expressed as a percentage of con siRNA group at 0 h. *P<0.05 vs. con siRNA group. (E) Determination of cell death by flow cytometry of Annexin V‑fluorescein isothiocyanate/PI staining in THP‑M cells transfected with con or p62 siRNA and then incubated with oxLDL (20 µg/ml) for the indicated times. Data are representatives of three independent experiments. **P<0.01 as indicated. <t>IL,</t> <t>interleukin;</t> oxLDL, oxidized low‑density lipoprotein; THP‑M, THP‑1‑derived macrophages; siRNA, small interfering RNA; con, control; PI, propidium iodide.
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human elisa il 18  (R&D Systems)
96
R&D Systems human elisa il 18
Figure 3. Silencing p62 reduces IL‑18 secretion and promotes cell survival of macrophages with prolonged oxLDL exposure. (A) THP‑M cells treated with oxLDL (20 µg/ml) for the indicated time following transfection with 50 nM con siRNA or p62 siRNA. Western blotting experiments were performed on total protein extracts using anti‑p62 antibody, and β‑actin expression was used as a loading control. The graph represents the values of p62 band intensity after normalization to β‑actin by densitometry. *P<0.05 vs. 24 h group. (B) The graph represents values of the SQSTM1 mRNA expression following normalization for β‑actin mRNA by reverse transcription‑quantitative polymerase chain reaction. *P<0.05 vs. 24 h group. (C) <t>ELISA</t> of secreted IL‑18 in the media of cells treated with con or p62 siRNA and 20 µg/ml oxLDL for 0, 12, 24, 48 and 72 h. *P<0.05 and **P<0.01 vs. 0 h; ##P<0.01 as indicated. (D) THP‑M cells were pretreated with con or p62 siRNA and then treated with 20 µg/ml oxLDL for the indicated times. Cell viability was analyzed by the MTT assay. Results are expressed as a percentage of con siRNA group at 0 h. *P<0.05 vs. con siRNA group. (E) Determination of cell death by flow cytometry of Annexin V‑fluorescein isothiocyanate/PI staining in THP‑M cells transfected with con or p62 siRNA and then incubated with oxLDL (20 µg/ml) for the indicated times. Data are representatives of three independent experiments. **P<0.01 as indicated. <t>IL,</t> <t>interleukin;</t> oxLDL, oxidized low‑density lipoprotein; THP‑M, THP‑1‑derived macrophages; siRNA, small interfering RNA; con, control; PI, propidium iodide.
Human Elisa Il 18, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human total il 18 il 1f4 quantikine elisa kit  (R&D Systems)
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R&D Systems human total il 18 il 1f4 quantikine elisa kit
Figure 3. Silencing p62 reduces IL‑18 secretion and promotes cell survival of macrophages with prolonged oxLDL exposure. (A) THP‑M cells treated with oxLDL (20 µg/ml) for the indicated time following transfection with 50 nM con siRNA or p62 siRNA. Western blotting experiments were performed on total protein extracts using anti‑p62 antibody, and β‑actin expression was used as a loading control. The graph represents the values of p62 band intensity after normalization to β‑actin by densitometry. *P<0.05 vs. 24 h group. (B) The graph represents values of the SQSTM1 mRNA expression following normalization for β‑actin mRNA by reverse transcription‑quantitative polymerase chain reaction. *P<0.05 vs. 24 h group. (C) <t>ELISA</t> of secreted IL‑18 in the media of cells treated with con or p62 siRNA and 20 µg/ml oxLDL for 0, 12, 24, 48 and 72 h. *P<0.05 and **P<0.01 vs. 0 h; ##P<0.01 as indicated. (D) THP‑M cells were pretreated with con or p62 siRNA and then treated with 20 µg/ml oxLDL for the indicated times. Cell viability was analyzed by the MTT assay. Results are expressed as a percentage of con siRNA group at 0 h. *P<0.05 vs. con siRNA group. (E) Determination of cell death by flow cytometry of Annexin V‑fluorescein isothiocyanate/PI staining in THP‑M cells transfected with con or p62 siRNA and then incubated with oxLDL (20 µg/ml) for the indicated times. Data are representatives of three independent experiments. **P<0.01 as indicated. <t>IL,</t> <t>interleukin;</t> oxLDL, oxidized low‑density lipoprotein; THP‑M, THP‑1‑derived macrophages; siRNA, small interfering RNA; con, control; PI, propidium iodide.
Human Total Il 18 Il 1f4 Quantikine Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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il 18 antibodies  (R&D Systems)
93
R&D Systems il 18 antibodies
Figure 3. Silencing p62 reduces IL‑18 secretion and promotes cell survival of macrophages with prolonged oxLDL exposure. (A) THP‑M cells treated with oxLDL (20 µg/ml) for the indicated time following transfection with 50 nM con siRNA or p62 siRNA. Western blotting experiments were performed on total protein extracts using anti‑p62 antibody, and β‑actin expression was used as a loading control. The graph represents the values of p62 band intensity after normalization to β‑actin by densitometry. *P<0.05 vs. 24 h group. (B) The graph represents values of the SQSTM1 mRNA expression following normalization for β‑actin mRNA by reverse transcription‑quantitative polymerase chain reaction. *P<0.05 vs. 24 h group. (C) <t>ELISA</t> of secreted IL‑18 in the media of cells treated with con or p62 siRNA and 20 µg/ml oxLDL for 0, 12, 24, 48 and 72 h. *P<0.05 and **P<0.01 vs. 0 h; ##P<0.01 as indicated. (D) THP‑M cells were pretreated with con or p62 siRNA and then treated with 20 µg/ml oxLDL for the indicated times. Cell viability was analyzed by the MTT assay. Results are expressed as a percentage of con siRNA group at 0 h. *P<0.05 vs. con siRNA group. (E) Determination of cell death by flow cytometry of Annexin V‑fluorescein isothiocyanate/PI staining in THP‑M cells transfected with con or p62 siRNA and then incubated with oxLDL (20 µg/ml) for the indicated times. Data are representatives of three independent experiments. **P<0.01 as indicated. <t>IL,</t> <t>interleukin;</t> oxLDL, oxidized low‑density lipoprotein; THP‑M, THP‑1‑derived macrophages; siRNA, small interfering RNA; con, control; PI, propidium iodide.
Il 18 Antibodies, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant human il 18bpa  (R&D Systems)
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Figure 3. Silencing p62 reduces IL‑18 secretion and promotes cell survival of macrophages with prolonged oxLDL exposure. (A) THP‑M cells treated with oxLDL (20 µg/ml) for the indicated time following transfection with 50 nM con siRNA or p62 siRNA. Western blotting experiments were performed on total protein extracts using anti‑p62 antibody, and β‑actin expression was used as a loading control. The graph represents the values of p62 band intensity after normalization to β‑actin by densitometry. *P<0.05 vs. 24 h group. (B) The graph represents values of the SQSTM1 mRNA expression following normalization for β‑actin mRNA by reverse transcription‑quantitative polymerase chain reaction. *P<0.05 vs. 24 h group. (C) <t>ELISA</t> of secreted IL‑18 in the media of cells treated with con or p62 siRNA and 20 µg/ml oxLDL for 0, 12, 24, 48 and 72 h. *P<0.05 and **P<0.01 vs. 0 h; ##P<0.01 as indicated. (D) THP‑M cells were pretreated with con or p62 siRNA and then treated with 20 µg/ml oxLDL for the indicated times. Cell viability was analyzed by the MTT assay. Results are expressed as a percentage of con siRNA group at 0 h. *P<0.05 vs. con siRNA group. (E) Determination of cell death by flow cytometry of Annexin V‑fluorescein isothiocyanate/PI staining in THP‑M cells transfected with con or p62 siRNA and then incubated with oxLDL (20 µg/ml) for the indicated times. Data are representatives of three independent experiments. **P<0.01 as indicated. <t>IL,</t> <t>interleukin;</t> oxLDL, oxidized low‑density lipoprotein; THP‑M, THP‑1‑derived macrophages; siRNA, small interfering RNA; con, control; PI, propidium iodide.
Recombinant Human Il 18bpa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human il 18 elisa kit  (Cusabio)
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Figure 3. Silencing p62 reduces IL‑18 secretion and promotes cell survival of macrophages with prolonged oxLDL exposure. (A) THP‑M cells treated with oxLDL (20 µg/ml) for the indicated time following transfection with 50 nM con siRNA or p62 siRNA. Western blotting experiments were performed on total protein extracts using anti‑p62 antibody, and β‑actin expression was used as a loading control. The graph represents the values of p62 band intensity after normalization to β‑actin by densitometry. *P<0.05 vs. 24 h group. (B) The graph represents values of the SQSTM1 mRNA expression following normalization for β‑actin mRNA by reverse transcription‑quantitative polymerase chain reaction. *P<0.05 vs. 24 h group. (C) <t>ELISA</t> of secreted IL‑18 in the media of cells treated with con or p62 siRNA and 20 µg/ml oxLDL for 0, 12, 24, 48 and 72 h. *P<0.05 and **P<0.01 vs. 0 h; ##P<0.01 as indicated. (D) THP‑M cells were pretreated with con or p62 siRNA and then treated with 20 µg/ml oxLDL for the indicated times. Cell viability was analyzed by the MTT assay. Results are expressed as a percentage of con siRNA group at 0 h. *P<0.05 vs. con siRNA group. (E) Determination of cell death by flow cytometry of Annexin V‑fluorescein isothiocyanate/PI staining in THP‑M cells transfected with con or p62 siRNA and then incubated with oxLDL (20 µg/ml) for the indicated times. Data are representatives of three independent experiments. **P<0.01 as indicated. <t>IL,</t> <t>interleukin;</t> oxLDL, oxidized low‑density lipoprotein; THP‑M, THP‑1‑derived macrophages; siRNA, small interfering RNA; con, control; PI, propidium iodide.
Human Il 18 Elisa Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 3. Silencing p62 reduces IL‑18 secretion and promotes cell survival of macrophages with prolonged oxLDL exposure. (A) THP‑M cells treated with oxLDL (20 µg/ml) for the indicated time following transfection with 50 nM con siRNA or p62 siRNA. Western blotting experiments were performed on total protein extracts using anti‑p62 antibody, and β‑actin expression was used as a loading control. The graph represents the values of p62 band intensity after normalization to β‑actin by densitometry. *P<0.05 vs. 24 h group. (B) The graph represents values of the SQSTM1 mRNA expression following normalization for β‑actin mRNA by reverse transcription‑quantitative polymerase chain reaction. *P<0.05 vs. 24 h group. (C) ELISA of secreted IL‑18 in the media of cells treated with con or p62 siRNA and 20 µg/ml oxLDL for 0, 12, 24, 48 and 72 h. *P<0.05 and **P<0.01 vs. 0 h; ##P<0.01 as indicated. (D) THP‑M cells were pretreated with con or p62 siRNA and then treated with 20 µg/ml oxLDL for the indicated times. Cell viability was analyzed by the MTT assay. Results are expressed as a percentage of con siRNA group at 0 h. *P<0.05 vs. con siRNA group. (E) Determination of cell death by flow cytometry of Annexin V‑fluorescein isothiocyanate/PI staining in THP‑M cells transfected with con or p62 siRNA and then incubated with oxLDL (20 µg/ml) for the indicated times. Data are representatives of three independent experiments. **P<0.01 as indicated. IL, interleukin; oxLDL, oxidized low‑density lipoprotein; THP‑M, THP‑1‑derived macrophages; siRNA, small interfering RNA; con, control; PI, propidium iodide.

Journal: Experimental and therapeutic medicine

Article Title: Oxidized low-density lipoprotein-induced p62/SQSTM1 accumulation in THP-1-derived macrophages promotes IL-18 secretion and cell death.

doi: 10.3892/etm.2017.5221

Figure Lengend Snippet: Figure 3. Silencing p62 reduces IL‑18 secretion and promotes cell survival of macrophages with prolonged oxLDL exposure. (A) THP‑M cells treated with oxLDL (20 µg/ml) for the indicated time following transfection with 50 nM con siRNA or p62 siRNA. Western blotting experiments were performed on total protein extracts using anti‑p62 antibody, and β‑actin expression was used as a loading control. The graph represents the values of p62 band intensity after normalization to β‑actin by densitometry. *P<0.05 vs. 24 h group. (B) The graph represents values of the SQSTM1 mRNA expression following normalization for β‑actin mRNA by reverse transcription‑quantitative polymerase chain reaction. *P<0.05 vs. 24 h group. (C) ELISA of secreted IL‑18 in the media of cells treated with con or p62 siRNA and 20 µg/ml oxLDL for 0, 12, 24, 48 and 72 h. *P<0.05 and **P<0.01 vs. 0 h; ##P<0.01 as indicated. (D) THP‑M cells were pretreated with con or p62 siRNA and then treated with 20 µg/ml oxLDL for the indicated times. Cell viability was analyzed by the MTT assay. Results are expressed as a percentage of con siRNA group at 0 h. *P<0.05 vs. con siRNA group. (E) Determination of cell death by flow cytometry of Annexin V‑fluorescein isothiocyanate/PI staining in THP‑M cells transfected with con or p62 siRNA and then incubated with oxLDL (20 µg/ml) for the indicated times. Data are representatives of three independent experiments. **P<0.01 as indicated. IL, interleukin; oxLDL, oxidized low‑density lipoprotein; THP‑M, THP‑1‑derived macrophages; siRNA, small interfering RNA; con, control; PI, propidium iodide.

Article Snippet: Interleukin-18 (IL-18) levels from culture media was measured with an ELISA kit from R&D Systems, Inc., (cat. no. 7620; Minneapolis, MN, USA), according to the manufacturer's instructions.

Techniques: Transfection, Western Blot, Expressing, Control, Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, MTT Assay, Flow Cytometry, Staining, Incubation, Small Interfering RNA